In November 2018, we celebrated Farhan's new job as an Assistant Professor at Hamad Bin Khalifa University in Qatar. While we were sad to see him leave, we are thrilled that he will be starting the next phase in his career. He is more than ready to take on the challenges of teaching and doing independent research.

On the 19th, 20th and 27th of May 2018, members of the lab volunteered to help introduce Drosophila research to children at Singapore's Science Centre.

James, Joses, Sangyu, and Quyen spent three weekend afternoons interacting with kids showing them Drosophila ​flies and experiments in person. Hundreds of children had fun looking at flies!

Four decades of memory genetics
We systematically reviewed over 30 years of Drosophila memory genetics. While replication is sparse, the replicated genes have high reproducibility. There is currently almost no evidence connecting genes into plasticity pathways.

Over Easter weekend 2018 we launched estimationstats.com on Twitter. There was a lot of interest, and the information spread to scientists in many different fields. The upsurge in usage provoked some server memory outages. Requests included a version in R, and an option that can tolerate very large sample sizes (which the swarmplot cannot handle). We're happy that people seem to find it helpful, and welcome feedback on what doesn't work and what does.

We mourn the loss of our colleague Katarina Chlebikova, a brilliant student and passionate scientist.

Katarina graduated with a degree in Biological Sciences from the University of Cambridge, doing her major project on learning and memory with Johan Alsiö. In the lab Jun - Sep 2013 she worked on Drosophila defense behaviors. Katarina went on to do her MSc at Edinburgh University before returning to Duke-NUS as a PhD student. She pursued her doctoral studies in the lab from Jan 2016. Tragically, Katarina passed away January 2018.

MS Word offers a huge range of advanced features. However, Google Docs is vastly superior for collaborative composition.

• Simultaneous collaborative editing. Using realtime version control, multiple people can edit the same Google Doc—even the same sentence—all at once. Because you can see where someone else is working, you can easily avoid writing over the text they are working on. No version control system, not Word Track Changes, Merge Documents, nor even git–GitHub offer such seamless collaborative editing.
• Threaded comments. In MS Word (at least the version I use on Mac), you cannot respond to a comment in a thread. All you can do is add a new comment, and your correspondent has to figure out which new comments are responses to their question. Threaded comments means that--rather than email back-and-forths--nearly all discussions about the text happen in context of the relevant text.
  
• All-to-all communication. For MS Word, if one person is handling the version control by email and Track Changes approvals, they must be involved in every editing decision. This puts more work on them. In Google Docs, sections can be more easily delegated to subgroups in the writing team, who (due to thread comments) can easily communicate with each other directly.
  
• Paperpile. If you use paid citation plugin Paperpile, anyone can insert a citation and anyone on the team can see the full citation. Paperpile is powerful citation software that is beautifully integrated into Chrome. See a paper on PubMed/ScienceDirect or a PDF? Click one button and it is now searchable and citable from within your GDoc.

Email and MS Word are technologies from 1971 and 1983, respectively. Their combination was never intended to be the final word in compositional version control. However, desktop word processors still do beat GDocs for typesetting; so I use GDocs for composition, followed by Apple Pages for typesetting the final manuscript.

Check out our recent preprint, a collaboration with the Jesuthasan group. We find that anion channelrhodopsins are effective inhibitors of zebrafish embryos, and that GtACR2 is not trafficked to the plasma membrane well.

Application Deadline: 27 January 2017

Apply here

Details
Prof Richard Baines of Manchester University and Dr Adam Claridge-Chang of A*STAR, Singapore are offering an exciting doctoral project to identify novel anticonvulsant compounds for the analysis and treatment of epilepsy. Doctoral research will be conducted in both Manchester (years 1 and 4) and Singapore (years 2 and 3). 

Current antiepileptic drugs are effective for only two-thirds of patients and are associated with significant side-effects. Better drugs are needed, but a lack of novel drug targets and good screening methods are significant barriers. The fruit fly (Drosophila melanogaster) offers the prospect to develop high-throughput screens to identify novel anticonvulsant compounds. Single gene mutations exist that increase the severity and duration of induced seizures in flies; seizures that respond well to treatment using existing antiepileptic drugs. This, and other studies, shows that the underlying biological basis of seizure in flies is remarkably similar to humans. 

In the first year, you will learn to work with Drosophila seizure mutants, using behavioral methods and calcium imaging to observe activity of central neurons. In the second year, you will implement advanced behavioral analysis methods (machine vision) to quantify seizure severity and dynamics. In the third year, you will use the behavioral analysis systems to screen for novel anticonvulsant compounds. These will include venom peptides that may block voltage-gated sodium channels and/or other excitatory ionic currents. In the final year, you will apply calcium-imaging and electrophysiology to determine mode-of-action for interesting anticonvulsant compounds identified.

This is an interdisciplinary research project that combines the quantitative analysis of seizure behaviours, drug-screening and in vivo physiology in Drosophila seizure models. As an ideal candidate, you would need to have an interest in epilepsy causes and treatments, along with the aptitude to learn a range of experimental techniques spanning neurogenetics, neurophysiology, computer-aided imaging and testing of compounds in whole-animal seizure screens. Both labs offer conscientious scientific mentorship, rigorous technical training and highly collaborative environments. 

Funding Notes
The project is available to UK/EU candidates. Funding covers fees (UK/EU rate) and stipend for four years. Overseas candidates can apply providing they can pay the difference in fees and are from an eligible country. Please check the website for information on eligibility. Candidates will be required to split their time between Manchester and Singapore, as outlined on our website. Applications should be submitted online and candidates should make direct contact with the Manchester supervisor to discuss their application directly.

References
1.Streit AK, Fan YN, Masullo L and Baines RA (2016) Calcium imaging of neuronal activity in Drosophila can identify anticonvulsive compounds. PLOS One DOI:10.1371/journal.pone.0148461  February 10, 2016. 
2.Giachello CNG and Baines RA (2015) Inappropriate neural activity during a sensitive period in embryogenesis results in persistent seizure-like behaviour. Curr. Biol. 25:2964-2968. 
3.Lin WH, He M and Baines RA (2015) Seizure suppression through manipulating splicing of a voltage-gated sodium channel. Brain 138:891-901. 
4. Alnabulsi S, Santina E, Russo I, Hussein B, Kadirvel M, Chadwick A, Bichenkova EV, Bryce RA, Nolan K, Demonacos C, Stratford IJ, Freeman S (2016) Non-symmetrical furan-amidines as novel leads for the treatment of cancer and malaria. Eur. J. Med. Chem.111:33–45. 
5. Emsley HC, Appleton RE, Whitmore CL, Jury F, Lamb JA, Martin JE, Ollier WE, de la Morandière KP, Southern KW and Allan SM (2014) Variations in inflammation-related genes may be associated with childhood febrile seizure susceptibility. Seizure 23:457-61

The anion channelrhodopsins from the algae Guillardia theta are highly effective at inhibiting neuronal activity in flies. Our manuscript is now available as a preprint.